Table II.

Binding of importin β fragments and mutants to Nup153 (895–1475) and to importin α

Importin βKd, Nup153 (895–1475)Kd, importin α
nMnM
WT1.0 ± 0.10.9 ± 0.1
1–3961.2 ± 0.1ND
m-N (1–396)160.1 ± 25.3ND
304–876152.2 ± 17.52.5 ± 0.2
m-C (304–876)NS4.3 ± 0.5
m-N91.3 ± 11.81.1 ± 0.1
L612D/F688A (m-C)1.2 ± 0.12.2 ± 0.3
m-N/m-C83.2 ± 9.12.1 ± 0.2
  • The binding reactions with importin α were performed in PBS containing 30 mg/ml BSA (see Materials and methods). NS, nonsaturated binding at importin β concentrations up to 800 nM.