Table I.

Amino acid sequences of the α chain COOH termini of wild-type IRs and deletion mutants

Receptor typeOligonucleotides for site-directed mutagenesis (upper strand)Amino acid sequence (COOH terminus of α chain)
B-type…713-VFVPRKTSSGTGAEDPRPS
Δ15′-CACTGGTGCCGAGCCTAGGCCATCTC-3′…713-VFVPRKTSSGTGAEPRPS
Δ25′-AGGCACTGGTGCCCCTAGGCCATCTC-3′…713-VFVPRKTSSGTGAPRPS
Δ35′-TTCAGGCACTGGTCCTAGGCCATCTC-3′…713-VFVPRKTSSGTGPRPS
Δ45′-CTCTTCAGGCACTCCTAGGCCATCTC-3′…713-VFVPRKTSSGTPRPS
Δ55′-AACCTCTTCAGGCCCTAGGCCATCTC-3′…713-VFVPRKTSSGPRPS
Δ65′-GAAAAACCTCTTCACCTAGGCCATCTC-3′…713-VFVPRKTSSPRPS
Δ75′-CAGAAAAACCTCTCCTAGGCCATCTC-3′…713-VFVPRKTSPRPS
Δ85′-CCCAGAAAAACCCCTAGGCCATCTC-3′…713-VFVPRKTPRPS
Δ95′-CGTCCCCAGAAAACCTAGGCCATCTC-3′…713-VFVPRKPRPS
Δ105′-TTTTCGTCCCCAGACCTAGGCCATCTC-3′…713-VFVPRPRPS
A-type…713-VFVPRPS
  • The table shows the nomenclature of the generated IR mutants, the DNA sequence of the upper strand oligonucleotides used in the QuikChange reaction (see Materials and methods), and the resulting amino acid sequences of the COOH terminus of the IR α chain. The 12 amino acids encoded by exon 11 and present in the B-type receptor are underlined.