Table I.

Antibodies used in the study

ProteinAntibodyTypeaReference/Source
IFT particle proteins
Complex A
IFT139139.1MCole et al., 1998
Complex B
IFT 172172.1MCole et al., 1998
IFT8181.1–4 (pooled)MCole et al., 1998
IFT5757.1MCole et al., 1998
FT52α-IFT52PDeane et al., 2001
IFT74/72α-IFT721, α-IFT722PThis paper
Kinesin II motor
FLA10 neckα-FLA10NPCole et al., 1998
FLA10 tailα-FLA10TPKozminski et al., 1995
Cytoplasmic dynein heavy chain
DHC1bα-DHC1bPPazour et al., 1999
Outer arm dynein
Intermediate chain
IC69α-IC69MSigma-Aldrich
Docking complex
DC83α-DC83PWakabayashi et al., 2001
Light chain
LC1α-LC1PBenashski et al., 1999
LC2α-LC2PPatel-King et al., 1997
Inner arm dynein
Light chain
Tctex-1α-Tctex-1PHarrison et al., 1998
Intermediate chain
IC140α-IC140Yang and Sale, 1998
RSPs
RSP1α-RSP1PWilliams et al., 1986
RSP3α-RSP3PWilliams et al., 1989
Tubulin
Acetylated α-tubulinMSigma-Aldrich
    β-TubulinPSilflow and Rosenbaum, 1981
Flagellar membrane glycoprotein
FMG-1α-FMG-1MBloodgood et al., 1986
Hemagglutinin epitope12CA5MField et al., 1988
Ovalbuminα-OvalbuminMSigma-Aldrich
  • a M, monoclonal; P, polyclonal.