Table I.

Quantification of ER distribution in EM pictures

Mother
 WTBud
 WTBud neck
 WTMother
 shs1ΔBud
 shs1ΔBud neck
 shs1Δ
% rough ER92 ± 372 ± 53 ± 693 ± 364 ± 891 ± 12
% smooth ER8 ± 328 ± 597 ± 67 ± 336 ± 89 ± 12
% surface
   covered48 ± 575 ± 1197 ± 562 ± 579 ± 1772 ± 27
  • EM images of medium-budded cells were analyzed using the software ImageJ 1.29. n = 8 for WT and 5 for shs1Δ. The presence of a ribbon of ribosome-free region close to the cortex indicates the presence of ER. Aligned ribosomes along the ER lumen (Fig. 4 C) indicate rough ER, whereas ribosomes unaligned and not touching each other indicate the presence of smooth ER.