Table I.

Localization dependencies of RNA-binding proteins

Mutant/proteinCAR-1CGH-1PGL-1
car-1(tm1753)-NormalUntested
car-1(RNAi)-NormalNormal
cgh-1(ok494)Bar-like structures-Untested
cgh-1(RNAi)Bar-like structures-Bar-like structuresa
cey-2(ok902)NormalNormalUntested
pgl-1(bn102)NormalNormal-
  • a In addition to localizing to bar-like structures, a significant fraction of PGL-1 remained concentrated on P-granules in cgh-1 (RNAi) gonads. Localization of PGL-1 was performed using a GFP fusion to PGL-1.

  • Gonad and embryo localizations of CAR-1, CGH-1, and PGL-1 in mutant worms. For cgh-1(RNAi) and cgh-1(ok492), localization was performed only in gonads due to sterility.