Table I.

Accumulation of centrosomal components in mitotic cells after Dgrip75 depletion in cultured cells

StainingControlDgrip75RNAi
BipolarMonopolar
%%%
γ-Tubulin100 (n = 100)100 (n = 124)95 (n = 66)
Dgrip8499 (n = 186)98 (n = 170)98 (n = 50)
Dgrip9191 (n = 103)100 (n = 55)100 (n = 48)
Dgrip75100 (n = 95)6 (n = 70)0 (n = 52)
Dgrip12878 (n = 71)3 (n = 93)nd
Dgrip16398 (n = 132)24 (n = 119)nd
Dgp71WD95 (n = 109)100 (n = 65)100 (n = 43)
  • Microtubules were stained with antibodies against α-tubulin and chromosomes with DAPI, whereas the spindle poles were immunostained with antibodies against γ-tubulin, Dgrip84, Dgrip91, Dgrip75, Dgrip128, Dgrip163, and Dgp71WD. For a correct interpretation, we need to keep in mind a decrease in the total amount of Dgrip128 and Dgrip163 in Dgrip75-depleted cells. n, number of prometaphases/metaphases analyzed. nd, not determined.