Table I.

GP values of immunostained adherent or detached PAECs

MarkerPAEC adherentPAEC detached
Cav110.392 ± 0.072 (n = 31)a0.383 ± 0.059 (n = 18)
pYCav110.482 ± 0.075 (n = 28)a,b0.419 ± 0.053 (n = 20)b
pFAK0.502 ± 0.067 (n = 33)N/A
CTxB10.430 ± 0.084 (n = 12)0.388 ± 0.054 (n = 16)c,d
CTxB (15 min)0.350 ± 0.056 (n = 13)
CTxB (30 min)0.283 ± 0.056 (n = 12)c
CTxB (120 min)0.243 ± 0.054 (n = 12)d
  • Adherent or detached PAECs were labeled with Laurdan and other markers and imaged as described in Materials and methods. GP images and confocal images at identical focal planes were compared pixel by pixel to determine the mean GP value ± SD (from n images) within pixels positively stained for Cav1, pYCav1, pFAK, and CTxB as indicated in Fig. 1. Pairs of superscripts indicate statistically significant differences between the two values sharing the same superscript.

  • 1 1–2 min in suspension if detached.

  • a,b P < 0.05.

  • c,d P < 0.001. Note that no significant differences were found in mean GP value of Cav1- or CTxB-stained membranes between adherent and detached cells.