Table II.

GP values of immunostained adherent or detached MEFs

Adherent MEFsWT MEFsCav1−/− MEFs
Cav10.382 ± 0.070 (n = 27)a
pYCav10.459 ± 0.076 (n = 24)a
pFAK0.422 ± 0.058 (n = 24)b0.297 ± 0.080 (n = 28)b
CTxB0.434 ± 0.069 (n = 11)0.442 ± 0.099 (n = 10)
Detached MEFs
CTxB (1 min)0.368 ± 0.061 (n = 13)αβ0.370 ± 0.032 (n = 12)
CTxB (15 min)0.306 ± 0.060 (n = 12)c0.365 ± 0.050 (n = 12)c
CTxB (30 min)0.270 ± 0.058 (n = 16)dα0.353 ± 0.040 (n = 12)d
CTxB (120 min)0.212 ± 0.086 (n = 14)eβ0.330 ± 0.037 (n = 12)e
  • MEFs on FN-coated coverslip for 4 h (adherent) or in suspension for 1–120 min (detached) were labeled and imaged as described in Materials and methods. Mean GP values ± the SD (from n images) were calculated for pixels stained positively for Cav1, pYCav1, pFAK, and CTxB. Pairs of footnotes denote statistically significant differences between the two values sharing the same footnote.

  • a,b P < 0.001.

  • c P < 0.05.

  • d,e P < 0.001. c–e compare WT MEFs to Cav1−/− MEFs.

  • α P < 0.05.

  • β P < 0.001. α and β compare the kinetics of CTxB-labeled domains in WT MEFs. No significant differences were found between adherent cells and cells immediately after detachment (1 min) for both WT and Cav1−/− MEFs, and no differences were found in the kinetics of CTxB-labeled domains in Cav1−/− MEFs.