Table II.

MT gliding velocities in the competitive motility assays with wild-type OSM-3 and OSM-3–G444E mutant versus kinesin-II

ParameterValue for each assay
Kinesin-II and OSM-3
    Kinesin-II (μM)0.000.290.561.001.672.503.334.004.444.715.00
    OSM-3 (μM)5.004.714.444.003.332.501.671.000.560.290.00
    Mole fraction of OSM-3 (%)100948980675033201160
    Mean velocity (μm/s)1.0750.9980.9060.7050.6150.5400.4760.4150.3470.3480.343
    Standard deviation (μm/s)0.1480.0830.0690.0520.0470.0250.0300.0510.0390.0360.052
    Count of MTs5563577459746358534930
Kinesin-II and OSM-3–G444E
    Kinesin-II (μM)0.000.290.561.001.672.503.334.004.444.715.00
    OSM-3–G444E (μM)5.004.714.444.003.332.501.671.000.560.290.00
    Mole fraction of OSM-3–G444E (%)100948980675033201160
    Mean velocity (μm/s)0.9730.9950.8690.7540.6840.6030.5640.5370.4930.4900.488
    Standard deviation (μm/s)0.1620.1800.1590.2040.0770.0960.0860.0520.0780.0760.043
    Count of MTs5566666873716166715067