Table II.

PCR primers and PCR conditions

PrimerProduct SizeAnnealing Temperature
bp°C
β myosin heavy chain
    5′-GCCAACACCAACCTGTCCAAGTTC-3′20566
    5′-TGCAAAGGCTCCAGGTCTGAGGGC-3′
MLC-2v
    5′-GCCAAGAAGCGGATAGAAGG-3′49955
    5′-CTGTGGTTCAGGGCTCAGTC-3′
Nkx-2.5
    5′-CAGTGGAGCTGGACAAAGCC-3′21655
    5′-TAGCGACGGTTCTGGAATTT-3′
GATA4
    5′-CTGTCATCTCACTATGGGCA-3′27560
    5′-CCAAGTCCGAGCAGGAATTT-3′
MEF2C
    5′-GGCCATGGTACACCGAGTACAACGAGC-3′40162
    5′-GGGGATCCCTGTGTTACCTGCACTTGG-3′
ALP
    5′-TTGAAACTCCAAAAGCTCAACACCA-3′45062
    5′-TCTCGTTATCCGAGTACCAGTCCC-3′
Bcrp1
    5′-CCATAGCCACAGGCCAAAGT-3′32756
    5′-GGGCCACATGATTCTTCCAC-3′
GAPDH
    5′-TTCTACCCACGGCAAGTTCAA-3′47063
    5′-GGATGACCTTGCCCACAGC-3′
β-actin
    5′-GGACCTGGCTGGCCGGGACC-3′58360
    5′-GCGGTGCACGATGGAGGGGC-3′