Table I.

Examples of differentially expressed genes in the three transgenic cell populations and their RT-qPCR confirmation

Ratio (KO/WT)
Ref Seq IDGeneLmnb1Rce1Icmt
NM_009964Crystallin, α B (Cryab)3.0 (5.8)2.7 (2.7)1.8 (4.4)
NM_007486Rho, GDP dissociation inhibitor β (Arhgdib)2.53.21.7
NM_010415Heparin-binding EGF-like growth factor (Hegfl)3.6 (6.1)3.0 (2.6)1.6 (4.9)
NM_019760Tumor differentially expressed 2 (Tde2)1.62.21.6
NM_007874Deleted in polyposis 1 (Dp1)1.94.01.2
NM_016774ATP synthase, H+ transporting mitochondrial F1 complex,
     β subunit (Atp5b)1.441.71.1
NM_010288Gap junction membrane channel protein α1 (Gja1)5.58.3 (8.4)0.47
XM_203363Slit homologue 3 (Slit3)0.390.520.83
NM_011333Chemokine (C-C motif) ligand 2 (Ccl2)0.410.330.66
NM_013496Cellular retinoic acid binding protein 1 (Crabp1)38 (50)0.070.3 (0.5)
NM_019390Lamin A (Lmna)2.8 (2.3)1.6 (1.3)(0.3)
NM_010722Lamin B2 (Lmnb2)(0.8)(1.9)(0.8)
  • Values in parentheses represent fold changes determined using qRT-PCR. KO, knockout.