Table I.

MT turnover during metaphase and preanaphase B in wild-type and stable GST–cyclin B–injected spindles in Drosophila embryos

Near poleEquator
EmbryoMitotic staget1/2Percentage of recoveryt1/2Percentage of recovery
ss
Wild typePreanaphase B8.6 ± 2.3 (n = 10)81.0 ± 14.3 (n = 10)5.8 ± 0.9 (n = 11)89.5 ± 6.8 (n = 11)
Wild typeAnaphase B2.8 ± 0.5 (n = 20)45.8 ± 11.2 (n = 20)5.3 ± 1.3 (n = 20)85.8 ± 8.8 (n = 20)
Stable cyclin B injectedPreanaphase B
     arrested7.8 ± 2.2 (n = 23)85.0 ± 8.13 (n = 23)6.57 ± 0.9 (n = 20)88.0 ± 8.6 (n = 20)
  • The t1/2 and percentage of recovery values for the pole and equator shown in the table are from experiments where the spindles were simultaneously bleached at the two regions.