Table II.

Plasmids

PlasmidsPlasmid DescriptionSource
pULP1-GFPYCplac22-ULP1-GFP-kanMXO. Kerscher
pRS426-ULP2pRS426-ULP2, ULP2 WT plasmid (screen)This study
YCp50-ULP1YCp50-ULP1, ULP1 WT plasmidLi and Hochstrasser, 2000
pULP1-YFPYCplac22-ULP1-YFPThis study
pULP1YCplac22-ULP1-9mycLi and Hochstrasser, 2003
YCplac22ulp1tsYCplac22-ulp1-3-33 (NAT, TRP1)O. Kerscher
pULP1Δ150-403YCplac22-ulp1Δ150-403-9mycThis study
pULP1Δ150-346YCplac22-ulp1Δ150-346-9mycThis study
pULP1Δ346-403YCplac22-ulp1Δ346-403-9mycThis study
pULP1-C478YCplac22-ulp1Δ2-144-9mycLi and Hochstrasser, 2003
pULP1-C275YCplac22-ulp1Δ2-346-9mycLi and Hochstrasser, 2003
pULP1-N417YCplac22-ulp1Δ418-621-9mycLi and Hochstrasser, 2003
pULP1-N346YCplac22-ulp1Δ347-621-9mycLi and Hochstrasser, 2003
pLGSD52μ, URA3 (lacZ without intron)Legrain and Rosbash, 1989
pJCR12μ, URA3 (lacZ in-frame in pre-mRNA)Legrain and Rosbash, 1989
pJCR512μ, URA3 (lacZ in-frame in spliced mRNA)Legrain and Rosbash, 1989
pJCR1mutBP2μ, URA3 (mutated branchpoint sequence)Rain and Legrain, 1997