Table I.

FRAP kinetic parameters

MotorDeffk*onkofft1/2CeqRecovery
μm2/ss−1s21s%
KLP10A-GFP2.3 ± 0.2696.9 ± 0.3
    Fast phase0.0370.201 ± 0.0293.50.08 ± 0.01
    Slow phase0.0340.031 ± 0.0009220.48 ± 0.01
NCD-GFP1.5 ± 0.1898.7 ± 0.5
    Fast phase0.0720.204 ± 0.01713.40.20 ± 0.01
    Slow phase0.0120.029 ± 0.0026240.23 ± 0.01
  • Values were obtained from curve fits of the two-state binding model (see Materials and methods) except for Deff (effective diffusion coefficient), which is from fits to a diffusion-binding model (Sprague et al., 2004). Ceq, protein bound at equilibrium. Value ± 95% confidence interval from the curve fit.