Table I.

Transport velocities of GFP tagged IFT proteins in wild-type and nephrocystin mutant animals

ReportersGenotypeMiddle segmentDistal segment
Mean velocity (μm/s)nt testf-testMean velocity (μm/s)n
Kinesin-2 motors
    KAP-1∷GFPWild type0.69 ± 0.09446None
nphp-10.69 ± 0.094200.490.4None
nphp-40.68 ± 0.093750.660.93None
nphp-1;nphp-40.68 ± 0.094230.770.46None
    OSM-3∷GFPWild type0.72 ± 0.071281.22 ± 0.14145
nphp-10.72 ± 0.07810.90.371.18 ± 0.1576
nphp-40.83 ± 0.15101<0.001<0.0011.16 ± 0.1455
nphp-1;nphp-40.81 ± 0.15213<0.001<0.0011.03 ± 0.14124
IFT particle A
    CHE-11∷GFPWild type0.71 ± 0.091191.13 ± 0.15119
nphp-10.72 ± 0.081310.90.221.19 ± 0.1595
nphp-40.74 ± 0.142520.09<0.0011.11 ± 0.16144
nphp-1;nphp-40.74 ± 0.133500.04<0.0011.06 ± 0.18125
IFT particle B
    OSM-6∷GFPWild type0.71 ± 0.082191.14 ± 0.16218
nphp-10.70 ± 0.09980.480.251.26 ± 0.1498
nphp-40.49 ± 0.06132<0.0010.021.07 ± 0.17104
nphp-1;nphp-40.47 ± 0.0757<0.0010.2ND-
nphp-4;kap-1None---None-
kap-1 bbs-7;nphp-4None---None-
osm-30.50 ± 0.07252<0.0010.22None-
osm-3;nphp-40.51 ± 0.06221<0.0010.006None-
    OSM-5∷GFPWild type0.71 ± 0.061251.06 ± 0.16132
nphp-10.72 ± 0.082810.5670.0041.07 ± 0.12163
nphp-40.75 ± 0.13336<0.001<0.0011.03 ± 0.14132
nphp-1;nphp-40.76 ± 0.15472<0.001<0.0011.05 ± 0.13233
DYF proteins
    DYF-1∷GFPWild type0.72 ± 0.081941.18 ± 0.14139
nphp-10.70 ± 0.09830.170.151.07 ± 0.1684
nphp-40.77 ± 0.16107<0.001<0.0011.06 ± 0.1680
nphp-1;nphp-40.76 ± 0.16167<0.001<0.0011.04 ± 0.16130
BBS proteins
    BBS-7∷GFPWild type0.68 ± 0.08231.14 ± 0.1656
nphp-10.70 ± 0.071100.190.631.15 ± 0.16148
nphp-40.82 ± 0.19100<0.001<0.0011.01 ± 0.2356
nphp-1;nphp-40.78 ± 0.1766<0.001<0.0011.02 ± 0.1431
  • IFT velocities in wild type and nephrocystin mutants. The velocities of many IFT components are altered in nphp-4 and nphp-1;nphp-4 but not nphp-1 animals. Distal segment velocities were similar to the wild type except for the slow OSM-6∷GFP rates. Mean difference was shown statistically by a t test, whereas statistical variance difference was shown by an analysis of variance test f-test.