Table II.

Yeast strains used in this study

NameGenotypeReference
CWY241SEY6210 ATG17-GFP∷HIS3Cheong et al. (2005)
JGY018SEY6210 ATG19-GFP∷TRP1This study
JGY053TN124 atg8Δ∷KAN PATG8GFP-ATG8∷URA3 atg5Δ∷TRP1This study
JGY054TN124 atg8Δ∷KAN PATG8GFP-ATG8∷URA3 atg2Δ∷TRP1This study
JGY056TN124 atg8Δ∷KAN PATG3GFP-ATG8∷URA3 pep4Δ∷TRP1This study
JGY057TN124 atg8Δ∷KAN PVPS30-ATG18GFP-ATG8∷URA3 pep4Δ∷TRP1This study
JGY058TN124 atg8Δ∷KAN PATG27GFP-ATG8∷URA3 pep4Δ∷TRP1This study
JGY059SEY6210 MLC2-GFP∷TRP1This study
JGY060SEY6210 CDC12-GFP∷TRP1This study
JGY069SEY6210 PCUP1APE1∷LEU2This study
JGY070JGY069 ATG19-GFP∷KANThis study
JGY071JGY069 atg8Δ∷KANThis study
JGY072JGY069 ATG9-GFP∷KANThis study
JGY075SEY6210 atg11Δ∷HIS3 GFP-ATG11∷TRP1This study
JGY087JGY069 Atg11-GFP∷KANThis study
JGY088JGY069 pep4Δ∷ KANThis study
JGY089JGY088 vps4Δ∷bleThis study
KTY97SEY6210 ATG9-GFP∷TRP1Xie et al. (2008)
KTY148SEY6210 ATG16-GFP∷KANXie et al. (2008)
PSY101SEY6210 ATG11-GFP∷HIS3He et al. (2006)
PSY143SEY6210 ATG1-GFP∷HIS3Reggiori et al. (2004)
SEY6210MATα ura3-52 leu2-3,112 his3-Δ200 trp1-Δ901 lys2-801 suc2-Δ9 mel GALRobinson et al. (1988)
TN124MATa leu2-3,112 trp1 ura3-52 pho8∷pho8Δ60 pho13∷LEU2Noda et al. (1995)
YZX246TN124 atg8Δ∷KAN PVPS30-ATG18GFP-ATG8∷URA3 TRP1This study
YZX247TN124 atg8Δ∷KAN PATG8GFP-ATG8∷URA3 TRP1Xie et al. (2008)
YZX251TN124 atg8Δ∷KAN PATG27GFP-ATG8∷URA3 TRP1Xie et al. (2008)
YZX254TN124 atg8Δ∷KAN PATG8GFP-ATG8∷URA3 pep4Δ∷TRP1Xie et al. (2008)
YZX257TN124 atg8Δ∷KAN PATG3GFP-ATG8∷URA3 TRP1Xie et al. (2008)
  • P, promoter.