Table I.

FC cell heteroplasmy as scored by FISH

Homoplasmic CWΔHeteroplasmic CWΔ:FLPΔHomoplasmic FLPΔNumber of cells examined
%%%
FC I
    Day 220100093
    Day 3255342109
    Day 5146450172
    Day 59124147116
    Day 10442553121
FC II
    Day 2237818132
    Day 2519503174
    Day 2842164193
    Day 3054640110
    Day 3249150156
    Day 6047944115
    Day 90224632122
    Day 120401248130
  • Cells for both fusion experiments (FC I and FC II) were scored as heteroplasmic CWΔ:FLPΔ if they carried appreciable FISH signal for both CW Δ-mtDNA (red) and FLP Δ-mtDNA (green) FISH probes. Cells having predominantly one signal were scored as homoplasmic. For example, the arrowheads in Fig. 6 B at day 120 show nucleoids that are in a clear minority (i.e. red in a predominantly green cell, green in a predominantly red cell). Both cells were considered to be essentially homoplasmic for the majority nucleoid population and were scored accordingly.