Table II.

CW Δ-mtDNA/FLP Δ-mtDNA colocalization in FC cells

ImageJ colocalizationNormalized
 colocalizationa
% ± SD%
WT58 ± 10 (n = 14)100
FC I
    Day 2218 ± 7 (n = 10)31
    Day 323 ± 3 (n = 10)5
    Day 517 ± 9 (n = 10)12
    Day 5911 ± 7 (n = 9)19
    Day 1044 ± 3 (n = 10)7
    Day 2742 ± 2 (n = 11)3
FC II
    Day 2216 ± 9 (n = 11)28
    Day 322 ± 4 (n = 10)4
    Day 602 ± 2 (n = 10)4
    Day 907 ± 4 (n = 9)12
    Day 1207 ± 6 (n = 8)12
    Day 1501 ± 2 (n = 10)2
  • Red and green images were thresholded to remove irrelevant pixel intensities (see Materials and methods) and the proportion of pixels carrying meaningful signal for both red and green were expressed as a percentage of the total. n = number of images analyzed for each timepoint (see Fig. 7 for examples).

  • a Colocalization expressed as a percentage of WT colocalization.