Table I.

Summary of speed and diffusion constants measured for kinesin-5 under different experimental conditions

Buffer (70 mM Pipes
 + KCl added)ATPADP
MDMSDMSD
v (nm/s)D (×103 nm2/s)nv (nm/s)D (×103 nm2/s)nD (×103 nm2/s)n
0 mM8.9 ± 0.10.67 ± 0.02779.1 ± 0.60.63 ± 0.06771.06 ± 0.0672
20 mM10.1 ± 0.31.34 ± 0.046211 ± 11.2 ± 0.2621.18 ± 0.0666
40 mM0.0 ± 0.402.38 ± 0.07500 ± 3 × 1042.6 ± 0.2813.3 ± 0.160
60 mM0.3 ± 0.63.3 ± 0.1480 ± 1 × 1063.8 ± 0.31233.8 ± 0.2103
80 mMNDNDNA3.1 ± 8.13.6 ± 0.3603.3 ± 0.3a90
60 mM (axonemes)23.0 ± 0.42.39 ± 0.074226.0 ± 1.31.8 ± 0.3421.29 ± 0.0954
  • NA, not applicable.

  • a Fit to first 5 s.