Table I.

KLP61F turnover during metaphase and anaphase B in spindles of D. melanogaster embryos

Near poleEquator
t1/2 in s% recoveryt1/2 in s% recovery
Preanaphase B8.8 ± 0.6 (n = 4)87 ± 4 (n = 4)5.9 ± 0.3 (n = 5)108 ± 7 (n = 5)
Anaphase B3.0 ± 0.6 (n = 5)51 ± 16a (n = 5)5.4 ± 0.2 (n = 4)79 ± 10 (n = 4)
  • a Note that this apparent decrease in recovery around the poles is due to the net loss of both tubulin and KLP61F in this region rather than being due to a stable population of KLP61F that turns over slowly (see text).