Table I.

Yeast strains used in this study

StrainDescriptive nameGenotypeSource or reference
CWY241WT (ATG17-GFP)SEY6210 ATG17-GFP∷HIS3(Cheong et al., 2005)
HCY107MKO (ATG17-GFP)YCY123 ATG17-GFP∷HIS3This study
HCY113MKO (ATG29 ATG17-GFP)YCY121 ATG17-GFP∷HIS3This study
HCY151MKO (ATG11 ATG17-GFP)YCY152 ATG17-GFP∷HIS3This study
JLY2atg12ΔSEY6210 atg12Δ∷kanMXThis study
PSY161atg6ΔSEY6210 atg6Δ∷HIS3This study
SEY6210WTMATa ura3–52 leu2–3,112 his3200 trp1901 lys2–801
     suc29 GAL(Robinson et al., 1988)
TVY1pep4ΔSEY6210 pep4Δ∷LEU2(Gerhardt et al., 1998)
WHY001atg1ΔSEY6210 atg1Δ∷HIS5(Shintani et al., 2002)
YCY26atg18ΔSEY6210 atg18Δ∷kanMXThis study
YCY32atg4Δ atg8ΔSEY6210 atg4Δ∷LEU2, atg8Δ∷HIS5This study
YCY121MKO (ATG29)SEY6210 atg1Δ, , , , , , , , , 10Δ,
     11Δ, 12Δ, 13Δ, 14Δ, 16Δ, 17Δ, 18Δ, 19Δ, 20Δ,
     21Δ, 23Δ, 24Δ, 27ΔThis study
YCY123MKOSEY6210 atg1Δ, , , , , , , , , 10Δ,
     11Δ, 12Δ, 13Δ, 14Δ, 16Δ, 17Δ, 18Δ, 19Δ, 20Δ,
     21Δ, 23Δ, 24Δ, 27Δ, 29ΔThis study
YCY124MKO (atg15Δ)YCY123 atg15Δ∷kanMXThis study
YCY125MKO (atg15Δ ape1Δ)YCY123 atg15Δ∷kanMX, ape1Δ∷HIS3This study
YCY126MKO (atg15Δ RFP-APE1)YCY123 atg15Δ∷kanMX, RFP-APE1∷LEU2This study
YCY137MKO (ATG3)SEY6210 atg1Δ, , , , , , , , 10Δ,
     11Δ, 12Δ, 13Δ, 14Δ, 16Δ, 17Δ, 18Δ, 19Δ, 20Δ,
     21Δ, 23Δ, 24Δ, 27Δ, 29ΔThis study
YCY152MKO (ATG11)YCY123 ATG11∷LYS2This study
  • WT, wild type.