Table II.

The effect of serine point mutations on the interaction between MBD-ank and the ankyrin-binding motif of sodium channels

GST-Nav 1.2 1,080–1,203 mutantskonkoffKD
M−1s−1s−1M
S1112A8.9 ± 0.2 ×1053.3 ± 0.4 × 10−33.7 ± 0.3 × 10−9
S1123A19.1 × 1053.4 × 10−31.8 × 10−9a
S1124A16.3 ± 3.2 × 1053.9 ± 0.3 × 10−32.5 ± 0.7 × 10−9
S1126A16.2 ± 14.4 × 1053.1 ± 0.8 × 10−32.6 ± 1.3 × 10−9
S1123-24A8.2 ± 2.6 × 1053.4 ± 0.2 × 10−34.5 ± 1.3 × 10−9
S1124-26A0.9 ± 1 × 1054.2 ± 1.6 × 10−31.6 ± 1.3 × 10−7
S1112-24-26ANANA2.6 ± 1.1 × 10−6b
  • Mean values ± SD are obtained from three to five experiments. NA, not applicable.

  • a Value from one experiment.

  • b KD values were calculated using steady-state analysis (see Materials and methods).