Table I.

Summary of results for all proteins analyzed in this study

NotesLipid boundLocalizationRatio FPM/FCytDτRange (63%)Range (95%)n
µm2/ssµmµm
GFPNoneNoneCytoplasmND31.36 ± 5.06NANANA13
PM-YFPNoneIntegralPMND0.79 ± 0.062.12 × 1021 ± 2.11 × 102143
GFP-PH123-MyoXNonePIP2 + PIP3PM1.15 ± 0.040.07 ± 0.026.96 ± 0.930.981.7014
PH-PLCδ1-GFPNonePIP2PM2.07 ± 0.121.24 ± 0.142.44 ± 0.272.464.2652
PH-PLCδ1R40L-GFPNoneNoneCytoplasmND21.47 ± 3.01NANANA21
GFP-PH-PH-PLCδ1NonePIP2PM3.49 ± 0.170.24 ± 0.0211.13 ± 1.832.293.9733
PH-PH-PLCδ1-GFPNonePIP2PM4.45 ± 0.400.28 ± 0.0311.08 ± 2.012.504.3330
PHR40L-PH-PLCδ1-GFPNonePIP2PM2.13 ± 0.120.34 ± 0.052.87 ± 0.261.402.4330
PH-PLCδ1-GFPCHO-M1PIP2PM1.66 ± 0.110.60 ± 0.102.21 ± 0.221.632.8215
GFP-PH-PH-PLCδ1CHO-M1PIP2PM2.99 ± 0.270.05 ± 0.017.03 ± 0.770.851.4813
GFP-GAP1mLYNoneCytoplasmND3.96 ± 0.66NANANA28
InsulinPIP3PM1.52 ± 0.081.33 ± 0.293.42 ± 0.443.025.2330
GFP-GAP1IP4BPLYPIP2PM2.55 ± 0.110.35 ± 0.043.50 ± 0.261.572.7240
InsulinPIP2 + PIP3PM4.44 ± 0.270.27 ± 0.026.46 ± 1.121.863.2246
GFP-GAP1IP4BP10 µM WmnNoneCytoplasmND4.62 ± 0.54NANANA19
GFP-GAP1IP4BP-ΔC2LYPIP2PM2.33 ± 0.130.41 ± 0.043.34 ± 0.501.652.8536
InsulinPIP2 + PIP3PM4.28 ± 0.270.49 ± 0.047.93 ± 1.782.794.8438
GFP-PH-GAP1IP4BPLYPIP2PM3.31 ± 0.180.59 ± 0.043.64 ± 0.382.073.5851
InsulinPIP2 + PIP3PM4.99 ± 0.370.70 ± 0.047.37 ± 1.063.215.5647
  • All experiments were in HEK cells unless otherwise stated. The localization of the protein (either plasma membrane [PM] or cytoplasm) is noted, along with the lipid bound (PtdIns(4,5)P2 or PtdIns(3,4,5)P3). The parameters are the ratio of fluorescence at the PM verses the cytoplasm (ratio of FPM/FCyt) as a crude index of membrane recruitment, lateral diffusion coefficient (D), and membrane dissociation time constant (τ). The range refers to the expected distance traveled on the membrane for the proportion of these proteins indicated in parentheses, and are estimated as √(2 × D × τ) for 63% and √(2 × D × 3 × τ) for 95%, respectively (Teruel and Meyer, 2000). NA, not applicable; ND, not determined.