Table II.

Quantification of protein accumulation during AZ assembly using NMJ in vivo imaging

Coexpressed proteins (A × B)Both A and BA before BB before A
BRP × DGluRIIABRP+/IIA+ 17/39 (44%)BRP+/IIA 0/39 (0%)BRP/IIA+ 22/39 (56%)
DLiprin-α × DGluRIIADLiprin-α+/IIA+ 16/39 (41%)DLiprin-α+/IIA 23/39 (59%)DLiprin-α/IIA+ 0/39 (0%)
BRP × DLiprin-αBRP+/DLiprin-α+ 8/31 (26%)BRP+/DLiprin-α 0/31 (0%)BRP/DLiprin-α+ 23/31 (74%)
DGluRIIA × CacIIA+/Cac+ 36/62 (58%)IIA+/Cac 21/62 (34%)IIA/Cac+ 5/62 (8%)
BRP × CacBRP+/Cac+ 42/62 (68%)BRP+/Cac 7/62 (11%)BRP/Cac+ 13/62 (21%)
  • Quantification of the relative accumulation of the indicated synaptic proteins at newly forming AZs (Δt = ∼12 h). A synaptic site was scored positive (+) or negative (−) for a specific protein depending on whether protein fluorescence signals exceeded the mean background level by >2.5-fold. For example, when comparing BRP and DGluRIIA (at synapses forming newly over 12 h), 44% were positive for both proteins, 0% for BRP only, and 56% for DGluRIIA only.