Table I.

Candidate targets of the RIDD pathway in mouse cells

Target nameUniGene IDLocalizationMean log2 (DTT/untreated)Decreased stability in DTT
hIre1RIre1−/−
Heparan-α-glucosaminide N-acetyltransferase (Hgsnat)Mm.28326Membrane (lysos.)−1.340.45Yes
Biogenesis of lysosome-related organelles complex-1, subunit 1 (Blos1)Mm.30118Cytosol−1.22−0.10Yes
Scavenger receptor class A, member 3 (Scara3)Mm.344095Membrane (ER/Golgi)−1.110.17Yes
PDGF receptor (Pdgfrb)Mm.4146Membrane (PM)−0.890.12Yes
Peripheral myelin protein (Pmp22)Mm.1237ECM−0.88−0.07Yes
Collagen, type VI, α1 (Col6)Mm.2509ECM−0.85−0.15Yes
Ephrin B2Mm.209813Membrane (PM)−1.06−0.14No
Microtubule-associated protein 7 domain containing 1 (MAP7d1)Mm.266716NA−0.690.10No
Splicing factor, Arg/Ser rich 3 (SRp20)Mm.6787Spliceosome−0.89−0.14No
Tripartite motif protein 16 (Trim16)Mm.117087Cytosol−0.730.12No
Galnt10Mm.271670Membrane (Golgi)−0.590.14Yes
Laminin B1 subunit 1Mm.172674ECM−1.400.08ND
Mannose receptor C type 2Mm.235616Membrane (PM)−1.14−0.17ND
Leu-rich repeat containing 8CMm.319847Membrane−0.99−0.17ND
Neural cell adhesion molecule 1Mm.4974Membrane (PM)−0.880.14ND
PDGFA-associated protein 1Mm.188851ECM−0.88−0.21ND
Syndecan 4Mm.3815Membrane (PM)−0.85−0.18ND
Homeo box B4Mm.3546Nucleus−0.84−0.03ND
Expressed sequence C79267Mm.30464NA−0.77−0.12ND
3-oxoacid CoA transferase 1Mm.13445ECM−0.77−0.02ND
Tripeptidyl peptidase IMm.20837ECM, mitoch., lysos.−0.74−0.12ND
RIKEN cDND 4932417H02Mm.209933NA−0.710.21ND
Pre–B cell leukemia transcription factor–interacting protein 1Mm.65906NA−0.690.33ND
Chondroitin sulfate proteoglycan 4 (CSP4)Mm.41329Membrane (PM)−0.680.28ND
SHANK-associated RH domain–interacting proteinMm.41463Cytosol−0.670.41ND
ST3 β-galactoside α-2,3-sialyltransferase 5sMm.38248Membrane (Golgi)−0.620.17ND
  • lysos., lysosomal; mitoch., mitochondria; NA, not applicable (no annotation available); PM, plasma membrane. Localization data are based on Gene Ontology annotations of the mouse genome. Log2 (DTT/untreated) RNA levels were determined by microarray analysis and represent the mean of three independent experiments. Stability data are shown in Fig. S2.