Table II.

Quantification of the mitotic defects in control or ISWI RNAi S2 cells at 4 da

ProphasePrometaphaseMetaphaseAnaphaseTelophaseMono/multipolar spindles
Control RNAi (GFP)7.9 ± 2.99.9 ± 0.223.3 ± 0.49.9 ± 1.341.1 ± 1.36.4 ± 3.4
ISWI RNAi (MRC015_H12)6.3 ± 0.411.2 ± 1.123.2 ± 4.53.4 ± 0.513.6 ± 1.97.3 ± 0.4
1b2b3b4bMitotic index
Control RNAi (GFP)1.0 ± 0.10003.3 ± 0.1
ISWI RNAi (MRC015_H12)6.7 ± 1.817.9 ± 5.62.9 ± 0.17.4 ± 6.53.0 ± 0.9
  • a Values represent the percentage of each mitotic phase or classified phenotype over the total number of mitotic cells. The cells at 4 d after dsRNA treatment were immunostained and counted under a microscope (more than 100 mitotic cells per sample). Errors represent SD from two independent experiments.

  • b Abnormal mitotic cells were classified into four phenotypes (Fig. 10 E).