Table II.

Quantification of chromatid segregation patterns in solo, snm, solo; snm, and controls

ChromatidsWTsolosnmsolo; snm
Anaphase I
XX\YY46 (100%)23 (31%)41 (58%)13 (14%)
XY\XY048 (65%)041 (43%)
XXY\Y02 (2.7%)4 (5.6%)19 (20%)
XYY\X01 (1.3%)4 (5.6%)16 (17%)
XXYY\O0022 (31%)6 (6%)
Total46747195
Metaphase II
XX67 (52%)15 (20%)NDND
YY61 (48%)14 (18%)NDND
XY047 (62%)NDND
X or XYY00NDND
Y or XXY00NDND
O or XXYY00NDND
Total12876NDND
  • Genotypes: WT, yw; solo, soloZ2-0198/Df(2L)A267; snm, snmZ3-2138/snmZ3-0317; and solo; snm, soloZ2-0198/soloZ2-3534; snmZ3-2138/snmZ3-0317. Chromatid constitution was determined primarily by numbers of 359 bp and AATAC repeat spots per pole or nucleus. For 359 bp repeat spots in WT and snm, some poles or nuclei exhibited only one 359 bp spot as a result of cohesion. In these cases, the karyotype determination was based on the number of X chromatids per chromosome revealed by DAPI staining. Percentages of each karyotype within each genotype are shown in parentheses.