Table II.

Genes identified in the whole genome RNAi screen that show high or low actin perimeter intensity staining or a failure to spread

Gene nameCGActin perimeter intensityCell spreadingCell number
LowHighActin/areaDecision tree
Profilin9553715,37685513,9030.14
Capping α1054015,38213527,3750.86
Cofilin425418315,203110.28
Capulet506123415,1491520.48
Unknown917559914,7865100.76
DIM4152312,77312,5841891.04
Unknown846519015,191503970.76
Unknown1088231315,06912190.72
HLH106852270314,689131,4780.68
ACC111981,62713,7668611,1181.36
Sar1707323915,1476200.72
Sly135393,33612,0717170.57
Cen1ba674210115,2829160.65
COP54841,95713,427280.94
α-COP796113315,249171,6400.31
β-COP62239a15,375246,8970.22
Syx5421475414,634352,7600.26
Garz84872,08313,304404800.86
  • CG, FlyBase gene ID. Failure to spread is shown by a high ratio of actin to area and rankings from the cofilin/capulet-trained decision tree. A high actin to area ratio indicates that a cell has a normal amount of actin but a small surface area, indicating a lack of cell spreading. Discrepancies between actin/area and decision tree rankings result from the decision tree using a different set of discriminating features, including actin distribution in the cell. Ranking reflects the position of the phenotype out of the entire list of ∼14,000 genes. Cell number indicates the normalized number of cells present after RNAi treatment, where 1.00 is the plate mean. Depletions resulting in a normalized cell number <0.1 were excluded. Data are from the initial screen, and do not include rescreen data. All genes in this table except profilin, capping protein α, cofilin, and capulet were rescreened with nonoverlapping RNAs where possible, and all phenotypes were repeated by visual inspection and computational analysis. See Materials Methods for description of analysis and rescreening methods.