Table I.

Depolymerization kinetics of spindle halves after onset of spindle disassembly

GenotypeRecovery frequencyShrinkage ratenP-value
F(rec) (s−1)R(hs) (µm × s−1)
23°C
Wild type0.00500.069 ± 0.00642NA
cdh1Δ nonhyperstable0.00540.051 ± 0.007210.085
cdh1Δ hyperstable0.00460.013 ± 0.00416<0.001
doc1Δ nonhyperstable0.00640.044 ± 0.006250.013
doc1Δ hyperstable0.00160.004 ± 0.00216<0.001
dbf2Δ nonhyperstable0.00790.029 ± 0.00419<0.001
dbf2Δ hyperstable0.0020.012 ± 0.00216<0.001
kip3Δ0.01020.044 ± 0.004390.002
dcc1Δ0.00780.043 ± 0.004400.003
ctf8Δ0.00820.041 ± 0.005390.008
she1Δ0.00530.048 ± 0.004450.008
mcm21Δ0.00590.060 ± 0.005420.31
37°C
Wild type0.00280.090 ± 0.00740NA
ipl1-3210.00450.052 ± 0.00436<0.001
kip3Δ0.01250.061 ± 0.009190.014
ipl1-321 kip3Δ0.00760.042 ± 0.01017<0.001
  • NA, not applicable. Cells with indicated the genotypes expressing GFP-Tub1 were imaged every 10 s, and the lengths of the spindle halves were measured at each time point. A recovery event was scored each time a spindle-half switched from shrinkage to growth. The shrinkage rate (R(hs)) represents the slope of the half-spindle lengths when plotted versus time (mean ± SEM). P-values were calculated using a model I analysis of variance test. Half-spindle shrinkage rates for each mutant were directly compared with the wild-type rates.