Table I.

Primer sequences used for quantitative and semiquantitative gene expression analysis

GeneSenseAntisenseLength
β-actin5′-CACGGCTGCTTCCAGCTC-3′5′-CACAGGACTCCATGCCCAG-3′134 bp
ADAMTS-55′-GACCGATGGCACTGAATGTA-3′5′-TGTACAGCTGGAGTTGTCTCCT-3′145 bp
Aggrecan5′-GTTGTCATCAGCACCAGCATC-3′5′-ACCACACAGTCCTCTCCAGC-3′509 bp
Axin-25′-TACCGGAGGATGCTGAAGGC-3′5′-CCACTGGCCGATTCTTCCTT-3′345 bp
Col1A15′-GCCCTGTCTGCTTCCTGTAA-3′5′-GGTTCAGTTTGGGTTGCTTG-3′104 bp
Col2A15′-CTGCTCGTCGCCGCTGTCCTT-3′5′-AAGGGTCCCAGGTTCTCCATC-3′432 bp
MMP-35′-CAACCGTGAGGAAAATCGATGCAG-3′5′-CGGCAAGATACAGATTCACGCTCAA-3′440 bp
MMP-135′-ACGGACCCATACAGTTTGAATACAGC-3′5′-CCATTTGTGGTGTGGGAAGTATCATC-3′360 bp
PCNA5′-GGAGAACTTGGAAATGGAAAC-3′5′-CTGCATTTAGAGTCAAGACCC-3′548 bp
Sox95′-GAACGCACATCAAGACGGAG-3′5′-TCTCGTTGATTTCGCTGCTC-3′631 bp
WNT-3A5′-CCATCCTCTGCCTCAAATTC-3′5′-TGGACAGTGGATATAGCAGCA-3′70 bp
FZD15′-TTCAGCAGCACATTCTGAGG-3′5′-CCTGCACACATTTTCCCTTT-3′154 bp
FZD25′-TCACGGTCTACATGATCAAA-3′5′-GCAACCTAAAAGTGAAATGG-3′266 bp
FZD35′-GGATGATCAAAGAAGCAAAG-3′5′-TTGAGCCGATGAGAACTACT-3′186 bp
FZD45′-GACTTTGGAAGGAACCTTTT-3′5′-TGAAACCCCGTCTCTACTAA-3′238 bp
FZD55′-GGTTTGGTGCAGGTGAATTT-3′5′-CTACAGCATGGGATAGGCACT-3′125 bp
FZD65′-TTCGGCAGCTCACTAGGATT-3′5′-CATCAGAAAATCTTGCCCAA-3′190 bp
FZD75′-CTGGAGTTCTTTGAAATGTGCT-3′5′-AAGGTTAGCTCCCATGATTCTC-3′133 bp
FZD85′-CGGTTTGGGTATTCTTAATG-3′5′-ACAGGGGTAAGCCTCTAAAC-3′215 bp
FZD95′-AACACAGAGAAGCTGGAGAA-3′5′-ACCACCAGTGACATGAAAAT-3′251 bp
FZD105′-AGAAACCCTTCAGTGCTACA-3′5′-AAAGTGTCTCTGCCAACCTA-3′205 bp