Table 2.

List of antibodies used in this study

TargetMono/polyclonalClone/referenceAntibody raised inSourceDilution for I.B.Dilution for I.F.
53BP1PolyclonalNB100-304RabbitNovus Biologicals1:800
β-ACTINMonoclonal8226MouseAbcam1:5,000
CHK1MonoclonalG4MouseSanta Cruz Biotechnology, Inc.1:100
CHK1 PhS345Monoclonal133D3RabbitCell Signaling Technology1:1,000
CHK2Polyclonalab8108RabbitAbcam1:2,000
CHK2 PhT68Polyclonal2661RabbitCell Signaling Technology1:500
DNA-PKcsMonoclonal18.2MouseThermo Fisher Scientific1:200
FibrillarinMonoclonalAFB01MouseUniversal Biologicals1:200
GFPPolyclonalA11122RabbitLife Technologies1:2,000
GFPMonoclonal7.1 + 13.1MouseRoche1:2,000
H2AXPolyclonalab11175RabbitAbcam1:4,000
γ-H2AXPolyclonal2577RabbitCell Signaling Technology1:5001:100
γ-H2AXMonoclonalJBW301MouseEMD Millipore1:1,000
H3K9me3Polyclonalab8898RabbitAbcam1:500
HA tagMonoclonal12CA5MouseHome made1:2,000
KAP-1Polyclonalab10483RabbitAbcam1:5,000
KAP-1 PhS824PolyclonalIHC-00073RabbitBethyl Laboratories, Inc.1:200
Ku70MonoclonalN3H10MouseAbcam1:200
Ku80Monoclonal111MouseThermo Fisher Scientific1:2,0001:100
Ku80 (for MEF)PolyclonalC-20GoatSanta Cruz Biotechnology, Inc.1:100
Lamin A/CPolyclonalN-18GoatSanta Cruz Biotechnology, Inc.1:200
NBS1PolyclonalNB100-143RabbitNovus Biologicals1:1,0001:700
Nucleolin/C23PolyclonalH-250RabbitSanta Cruz Biotechnology, Inc.1:100
p53Monoclonal1C12MouseCell Signaling Technology1:900
PCNAPolyclonalab18197RabbitAbcam1:1,000
RAD51PolyclonalH-92RabbitSanta Cruz Biotechnology, Inc.1:200
RPA2/RPA32Monoclonal9H8MouseAbcam1:7501:250
XLFPolyclonalab33499RabbitAbcam1:500
XRCC4Polyclonalab145RabbitAbcam1:2,000
  • Minus signs indicate that the antibody was not used for this application in this study. PCNA, proliferating cell nuclear antigen; I.B., immunoblotting; I.F., immunofluorescence.