Yongrong Liao et al. show how UBAP2L facilitates assembly and stability of mature NPCs at the nuclear envelope during early interphase to ensure efficient nuclear transport, adaptation to nutrient stress, and cellular proliferative capacity of human cells.

This study demonstrates the critical role of BRG1/SMARCA4 for oligodendrocyte precursor cell differentiation and remyelination and highlights the unique dual function of BRG1/SMARCA4 in promoting oligodendrocyte differentiation and inhibiting proneuronal programs through epigenetic regulation with EED/PRC2 complexes, thereby ensuring oligodendrocyte lineage commitment and differentiation.

The findings reveal how calcium regulates migrasome formation and propose a sequential interaction model involving Syt1 and Tetraspanins in the formation and stabilization of migrasomes. The research uncovers a pivotal step that facilitates the transition from tension-induced swelling to the establishment of a stable migrasome.

Centromeric DNA regions are highly diverse and evolve rapidly. Chen et al. investigate how the correct balance of centromeric transcripts from mouse stem cells affects chromatin recruitment of CENPC and propose a model where structural RNA features, rather than sequence motifs, are key for RNA–protein interactions.

Hegde et al. report that extracellular Caspase-1 functions as a chemotactic factor. This work solves the long-standing question of the cue(s) that coax the migration of HFSCs into the epidermis during the wound healing response and inflammatory skin conditions.

Li et al. reveal that liquid–liquid phase separation of host fragile X–related (FXR) family proteins drives clustering of replication organelles of SARS-CoV-2 via specific interaction with viral nonstructural protein Nsp3. FXRs further recruit translation machinery to facilitate viral protein translation for efficient viral replication.

Maib et al. present a toolbox of recombinant biosensors for the multiplex and super-resolution detection of phosphoinositides, a small family of signaling lipids. Detection of these crucial lipids in fixed cells and tissues will enable researchers to address key outstanding questions in cell biology.