The Journal of Cell Biology Articles

Mammalian Rif1 contributes to replication stress survival and homology-directed repair

Buonomo, S. B.C., Wu, Y., Ferguson, D., de Lange, T. — Mon, 02 Nov 2009 10:42:11 PST

Multifunctional protein Rif1 accumulates at stalled replication forks to facilitate DNA repair during S phase.

Ase1/Prc1-dependent spindle elongation corrects merotely during anaphase in fission yeast

Courtheoux, T., Gay, G., Gachet, Y., Tournier, S. — Mon, 02 Nov 2009 10:42:11 PST

The tug of war that ensues when a kinetochore binds microtubules from both spindle poles is resolved by Ase1/Prc1.

Membrane proteins Bqt3 and -4 anchor telomeres to the nuclear envelope to ensure chromosomal bouquet formation

Mon, 02 Nov 2009 10:42:11 PST

A screen identifies two more bouquet proteins required for meiotic telomere clustering: Bqt4 anchors the telomeres, whereas Bqt3 protects Bqt4 from degradation.

MCL-1-dependent leukemia cells are more sensitive to chemotherapy than BCL-2-dependent counterparts

Brunelle, J. K., Ryan, J., Yecies, D., Opferman, J. T., Letai, A. — Mon, 02 Nov 2009 10:42:11 PST

Cancer-expressed anti-apoptotic protein BCL-2 is stable, whereas MCL-1 undergoes proteasome-dependent degradation in response to chemotherapeutic agents.

Cohesin SMC1{beta} protects telomeres in meiocytes

Mon, 19 Oct 2009 10:02:31 PDT

Telomeres fail to attach to the nuclear envelope and lose structural integrity in cells lacking SMC1β.

Ubiquilin and p97/VCP bind erasin, forming a complex involved in ERAD

Mon, 19 Oct 2009 10:02:31 PDT

Loss of ubiquilin or erasin activates ER stress, increases accumulation of polyubiquitinated proteins, and shortens lifespan in worms.

Temporal regulation of Drosophila IAP1 determines caspase functions in sensory organ development

Koto, A., Kuranaga, E., Miura, M. — Mon, 19 Oct 2009 10:02:31 PDT

Caspase activation is regulated by the turnover of E3 ubiquitin ligase, DIAP1, and depends on cell type and maturity.

Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors

Mon, 19 Oct 2009 10:02:31 PDT

Pex3 links peroxisome formation and inheritance. By binding to class V myosin, biogenesis protein Pex3 also directs the organelles into daughter cells.

AP-1 and KIF13A coordinate endosomal sorting and positioning during melanosome biogenesis

Mon, 19 Oct 2009 10:02:31 PDT

The clathrin adaptor protein AP-1 and the motor KIF13A work together to deliver cargo into maturing melanosomes.

Spred2 interaction with the late endosomal protein NBR1 down-regulates fibroblast growth factor receptor signaling

Mardakheh, F. K., Yekezare, M., Machesky, L. M., Heath, J. K. — Mon, 19 Oct 2009 10:02:31 PDT

Neighbor of BRCA1 (NBR1) suppresses growth factor responses by redirecting activated receptors to lysosomes for degradation.

PIKfyve regulates CaV1.2 degradation and prevents excitotoxic cell death

Tsuruta, F., Green, E. M., Rousset, M., Dolmetsch, R. E. — Mon, 19 Oct 2009 10:02:31 PDT

Neuronal Ca levels are regulated by glutamate receptor activation, which recruits PIKfyve to voltage-gated Ca channels, prompting their degradation.

Postsynaptic regulation of synaptic plasticity by synaptotagmin 4 requires both C2 domains

Barber, C. F., Jorquera, R. A., Melom, J. E., Littleton, J. T. — Mon, 19 Oct 2009 10:02:31 PDT

Analogous to synaptotagmin 1, a calcium-sensitive regulator of presynaptic vesicle fusion, synaptotagmin 4 needs both of its calcium-binding sites to regulate synaptic plasticity via postsynaptic retrograde signaling.

Molecular mechanisms that enhance synapse stability despite persistent disruption of the spectrin/ankyrin/microtubule cytoskeleton

Massaro, C. M., Pielage, J., Davis, G. W. — Mon, 05 Oct 2009 12:07:45 PDT

Neuromuscular junctions crippled by a disrupted microtubule cytoskeleton are rescued by stress-induced activation of MAPK-JNK-Fos signaling.

Cdc42 antagonizes Rho1 activity at adherens junctions to limit epithelial cell apical tension

Warner, S. J., Longmore, G. D. — Mon, 05 Oct 2009 12:07:45 PDT

Rho promotes actomyosin contractility during epithelial cell remodeling, but Cdc42 keeps the epithelium in shape by limiting RhoA activity.

Electron-tomographic analysis of intraflagellar transport particle trains in situ

Mon, 05 Oct 2009 12:07:45 PDT

Ultrastructural study of Chlamydomonas cilia shows that anterograde IFT particles form trains that are long and narrow, while retrograde IFT form short, compact particle trains.

Entropic organization of interphase chromosomes

Cook, P. R., Marenduzzo, D. — Mon, 21 Sep 2009 08:52:26 PDT

Nonspecific forces, in addition to biophysical properties, lead to the careful arrangement of chromosomes in the nucleus.

Nucleotide excision repair-induced H2A ubiquitination is dependent on MDC1 and RNF8 and reveals a universal DNA damage response

Mon, 21 Sep 2009 08:52:26 PDT

The epigenetic mark indicative of DNA UV damage or double-strand breaks is achieved via a common pathway regardless of the cause of damage.

Ribosome stalk assembly requires the dual-specificity phosphatase Yvh1 for the exchange of Mrt4 with P0

Lo, K.-Y., Li, Z., Wang, F., Marcotte, E. M., Johnson, A. W. — Mon, 21 Sep 2009 08:52:26 PDT

The step by step assembly process from preribosome in the nucleus to translation-competent 60S ribosome subunit in the cytoplasm is revealed (also see Kemmler et al. in this issue).

Yvh1 is required for a late maturation step in the 60S biogenesis pathway

Kemmler, S., Occhipinti, L., Veisu, M., Panse, V. G. — Mon, 21 Sep 2009 08:52:26 PDT

The step by step assembly process from preribosome in the nucleus to translation-competent 60S ribosome subunit in the cytoplasm is revealed (also see Lo et al. in this issue).

Impaired dense core vesicle maturation in Caenorhabditis elegans mutants lacking Rab2

Mon, 21 Sep 2009 08:52:27 PDT

Uncoordinated movement in Rab2 mutants is caused by impaired retention of cargo on dense core vesicles, not by defective synaptic vesicle release. (Also see the companion article by Sumakovic et al. in this issue.)

UNC-108/RAB-2 and its effector RIC-19 are involved in dense core vesicle maturation in Caenorhabditis elegans

Mon, 21 Sep 2009 08:52:27 PDT

Tropomodulin1 is required for membrane skeleton organization and hexagonal geometry of fiber cells in the mouse lens

Nowak, R. B., Fischer, R. S., Zoltoski, R. K., Kuszak, J. R., Fowler, V. M. — Mon, 21 Sep 2009 08:52:27 PDT

The spectrin–actin network is disrupted in Tmod1 mutants, disturbing fiber cell morphology, and disordering lens cell organization.