The Journal of Cell Biology Articles

Quantitative analysis of chromatin compaction in living cells using FLIM-FRET

Lleres, D., James, J., Swift, S., Norman, D. G., Lamond, A. I. — Mon, 16 Nov 2009 10:11:50 PST

FRET analysis of cell lines expressing fluorescently tagged histones on separate nucleosomes demonstrates that variations in chromosome compaction occur during mitosis.

Lte1 contributes to Bfa1 localization rather than stimulating nucleotide exchange by Tem1

Geymonat, M., Spanos, A., de Bettignies, G., Sedgwick, S. G. — Mon, 16 Nov 2009 10:11:50 PST

Reevaluation of Lte1’s involvement in the mitotic exit network reveals that it is involved in localizing Bfa1 to the spindle pole body but does not function as a GEF for Tem1.

Capacity for stochastic self-renewal and differentiation in mammalian spermatogonial stem cells

Mon, 16 Nov 2009 10:11:50 PST

Spermatogonial stem cells have an innate ability to choose, with constant probability, between different fates independently of cues from the microenvironment.

Membrane expansion alleviates endoplasmic reticulum stress independently of the unfolded protein response

Schuck, S., Prinz, W. A., Thorn, K. S., Voss, C., Walter, P. — Mon, 16 Nov 2009 10:11:50 PST

Increasing the size of the ER by lipid synthesis helps the cell deal with ER stress.

Adenovirus RID-{alpha} activates an autonomous cholesterol regulatory mechanism that rescues defects linked to Niemann-Pick disease type C

Cianciola, N. L., Carlin, C. R. — Mon, 16 Nov 2009 10:11:50 PST

Viral subversion of cholesterol homeostasis provides insights into sterol trafficking, autophagy, and lysosomal storage diseases.

The CEACAM1 N-terminal Ig domain mediates cis- and trans-binding and is essential for allosteric rearrangements of CEACAM1 microclusters

Mon, 16 Nov 2009 10:11:50 PST

Structural analyses reveal that oligomerization between cell adhesion molecules in the same membrane is influenced by their interactions across opposing membranes (see also in this issue the accompanying paper by Müller et al.).

Homophilic adhesion and CEACAM1-S regulate dimerization of CEACAM1-L and recruitment of SHP-2 and c-Src

Muller, M. M., Klaile, E., Vorontsova, O., Singer, B. B., Obrink, B. — Mon, 16 Nov 2009 10:11:50 PST

The monomer/dimer equilibrium of adhesion molecule CEACAM1-L is regulated by binding between opposing membranes, which in turn controls cytoplasmic enzyme binding and signaling (see also in this issue the accompanying paper by Klaile et al.).

Mammalian Rif1 contributes to replication stress survival and homology-directed repair

Buonomo, S. B.C., Wu, Y., Ferguson, D., de Lange, T. — Mon, 02 Nov 2009 10:42:11 PST

Multifunctional protein Rif1 accumulates at stalled replication forks to facilitate DNA repair during S phase.

Ase1/Prc1-dependent spindle elongation corrects merotely during anaphase in fission yeast

Courtheoux, T., Gay, G., Gachet, Y., Tournier, S. — Mon, 02 Nov 2009 10:42:11 PST

The tug of war that ensues when a kinetochore binds microtubules from both spindle poles is resolved by Ase1/Prc1.

Membrane proteins Bqt3 and -4 anchor telomeres to the nuclear envelope to ensure chromosomal bouquet formation

Mon, 02 Nov 2009 10:42:11 PST

A screen identifies two more bouquet proteins required for meiotic telomere clustering: Bqt4 anchors the telomeres, whereas Bqt3 protects Bqt4 from degradation.

MCL-1-dependent leukemia cells are more sensitive to chemotherapy than BCL-2-dependent counterparts

Brunelle, J. K., Ryan, J., Yecies, D., Opferman, J. T., Letai, A. — Mon, 02 Nov 2009 10:42:11 PST

Cancer-expressed anti-apoptotic protein BCL-2 is stable, whereas MCL-1 undergoes proteasome-dependent degradation in response to chemotherapeutic agents.

Cohesin SMC1{beta} protects telomeres in meiocytes

Mon, 19 Oct 2009 10:02:31 PDT

Telomeres fail to attach to the nuclear envelope and lose structural integrity in cells lacking SMC1β.

Ubiquilin and p97/VCP bind erasin, forming a complex involved in ERAD

Mon, 19 Oct 2009 10:02:31 PDT

Loss of ubiquilin or erasin activates ER stress, increases accumulation of polyubiquitinated proteins, and shortens lifespan in worms.

Temporal regulation of Drosophila IAP1 determines caspase functions in sensory organ development

Koto, A., Kuranaga, E., Miura, M. — Mon, 19 Oct 2009 10:02:31 PDT

Caspase activation is regulated by the turnover of E3 ubiquitin ligase, DIAP1, and depends on cell type and maturity.

Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors

Mon, 19 Oct 2009 10:02:31 PDT

Pex3 links peroxisome formation and inheritance. By binding to class V myosin, biogenesis protein Pex3 also directs the organelles into daughter cells.

AP-1 and KIF13A coordinate endosomal sorting and positioning during melanosome biogenesis

Mon, 19 Oct 2009 10:02:31 PDT

The clathrin adaptor protein AP-1 and the motor KIF13A work together to deliver cargo into maturing melanosomes.

Spred2 interaction with the late endosomal protein NBR1 down-regulates fibroblast growth factor receptor signaling

Mardakheh, F. K., Yekezare, M., Machesky, L. M., Heath, J. K. — Mon, 19 Oct 2009 10:02:31 PDT

Neighbor of BRCA1 (NBR1) suppresses growth factor responses by redirecting activated receptors to lysosomes for degradation.

PIKfyve regulates CaV1.2 degradation and prevents excitotoxic cell death

Tsuruta, F., Green, E. M., Rousset, M., Dolmetsch, R. E. — Mon, 19 Oct 2009 10:02:31 PDT

Neuronal Ca levels are regulated by glutamate receptor activation, which recruits PIKfyve to voltage-gated Ca channels, prompting their degradation.

Postsynaptic regulation of synaptic plasticity by synaptotagmin 4 requires both C2 domains

Barber, C. F., Jorquera, R. A., Melom, J. E., Littleton, J. T. — Mon, 19 Oct 2009 10:02:31 PDT

Analogous to synaptotagmin 1, a calcium-sensitive regulator of presynaptic vesicle fusion, synaptotagmin 4 needs both of its calcium-binding sites to regulate synaptic plasticity via postsynaptic retrograde signaling.

Molecular mechanisms that enhance synapse stability despite persistent disruption of the spectrin/ankyrin/microtubule cytoskeleton

Massaro, C. M., Pielage, J., Davis, G. W. — Mon, 05 Oct 2009 12:07:45 PDT

Neuromuscular junctions crippled by a disrupted microtubule cytoskeleton are rescued by stress-induced activation of MAPK-JNK-Fos signaling.

Cdc42 antagonizes Rho1 activity at adherens junctions to limit epithelial cell apical tension

Warner, S. J., Longmore, G. D. — Mon, 05 Oct 2009 12:07:45 PDT

Rho promotes actomyosin contractility during epithelial cell remodeling, but Cdc42 keeps the epithelium in shape by limiting RhoA activity.

Electron-tomographic analysis of intraflagellar transport particle trains in situ

Mon, 05 Oct 2009 12:07:45 PDT

Ultrastructural study of Chlamydomonas cilia shows that anterograde IFT particles form trains that are long and narrow, while retrograde IFT form short, compact particle trains.