The Journal of Cell Biology Reports

{gamma}-Tubulin ring complexes regulate microtubule plus end dynamics

Mon, 02 Nov 2009 10:42:10 PST

Independently of their nucleation activity, -tubulin ring complex proteins localize along microtubules, limiting catastrophe events during interphase.

From autoinhibition to inhibition in trans: the Raf-1 regulatory domain inhibits Rok-{alpha} kinase activity

Mon, 02 Nov 2009 10:42:10 PST

The mechanism by which Raf-1 antagonizes Rok- to promote migration and tumorigenesis is revealed.

Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1

Mon, 02 Nov 2009 10:42:10 PST

Cripto-1 associates with Notch1 in the endoplasmic reticulum and Golgi to enhance Notch1 localization to lipid rafts and its maturation.

Ana3 is a conserved protein required for the structural integrity of centrioles and basal bodies

Stevens, N. R., Dobbelaere, J., Wainman, A., Gergely, F., Raff, J. W. — Mon, 02 Nov 2009 10:42:10 PST

Centriolar protein Ana3 is essential for basal body formation, not centriole duplication, as previously thought.

Lateral transport of Smoothened from the plasma membrane to the membrane of the cilium

Milenkovic, L., Scott, M. P., Rohatgi, R. — Mon, 02 Nov 2009 10:42:10 PST

A distinct pathway for ligand-stimulated lateral trafficking of the hedgehog protein Smoothened into cilia is described.

Heterogeneity in the physiological states and pharmacological responses of differentiating 3T3-L1 preadipocytes

Loo, L.-H., Lin, H.-J., Singh, D. K., Lyons, K. M., Altschuler, S. J., Wu, L. F. — Mon, 02 Nov 2009 10:42:11 PST

A systems biology–based analysis shows that differentiating adipocytes look very different at the single-cell level and form distinct cellular subpopulations.

Differential regulation of telomere and centromere cohesion by the Scc3 homologues SA1 and SA2, respectively, in human cells

Canudas, S., Smith, S. — Mon, 19 Oct 2009 10:02:31 PDT

In vertebrates, two Scc3 homologues control sister chromatid cohesion of distinct chromosomal regions.

Keratins regulate protein biosynthesis through localization of GLUT1 and -3 upstream of AMP kinase and Raptor

Vijayaraj, P., Kroger, C., Reuter, U., Windoffer, R., Leube, R. E., Magin, T. M. — Mon, 19 Oct 2009 10:02:31 PDT

Removal of the entire keratin family of intermediate filament proteins from embryonic epithelia has surprising implications for mTOR signaling.

DNA replication times the cell cycle and contributes to the mid-blastula transition in Drosophila embryos

McCleland, M. L., Shermoen, A. W., O'Farrell, P. H. — Mon, 05 Oct 2009 12:07:44 PDT

Deletion of S phase disrupts mitotic timing in maternally regulated cycles, but it doesn't alter the cell cycle once zygotic transcription has begun.

Tim-Tipin dysfunction creates an indispensible reliance on the ATR-Chk1 pathway for continued DNA synthesis

Smith, K. D., Fu, M. A., Brown, E. J. — Mon, 05 Oct 2009 12:07:44 PDT

Increased amounts of single-stranded DNA accumulate at replication forks when Tim–Tipin isn't around, activating an ATR-mediated DNA damage response necessary for DNA replication to proceed.

INTS3 controls the hSSB1-mediated DNA damage response

Mon, 05 Oct 2009 12:07:44 PDT

MISE is identified as a component of the Integrator complex required for DNA repair.

An eIF4E-binding protein regulates katanin protein levels in C. elegans embryos

Li, W., DeBella, L. R., Guven-Ozkan, T., Lin, R., Rose, L. S. — Mon, 05 Oct 2009 12:07:44 PDT

SPN2 represses katanin translation to prevent mitotic defects (independently of ubiquitin-mediated katanin degradation).

Relative contributions of chromatin and kinetochores to mitotic spindle assembly

O'Connell, C. B., Loncarek, J., Kalab, P., Khodjakov, A. — Mon, 05 Oct 2009 12:07:45 PDT

It's the kinetochores, not the DNA, that initiate spindle assembly.

Coupled myosin VI motors facilitate unidirectional movement on an F-actin network

Sivaramakrishnan, S., Spudich, J. A. — Mon, 05 Oct 2009 12:07:45 PDT

A combination of experimentation and modeling reveal that multiple myosin VI molecules coordinately transport cargo over the actin filament network.

Glyburide inhibits the Cryopyrin/Nalp3 inflammasome

Mon, 05 Oct 2009 12:07:45 PDT

Glyburide, a sulfonylurea drug commonly used to treat type 2 diabetes, shuts down IL-1β secretion by preventing Cyropyrin activation.

Autophagy promotes synapse development in Drosophila

Shen, W., Ganetzky, B. — Mon, 05 Oct 2009 12:07:45 PDT

The ubiquitin–proteosome and autophagy pathways work together to regulate synaptic growth and plasticity in response to environmental conditions.

Intraflagellar transport particle size scales inversely with flagellar length: revisiting the balance-point length control model

Engel, B. D., Ludington, W. B., Marshall, W. F. — Mon, 05 Oct 2009 12:07:45 PDT

Chlamydomonas reinhardtii IFT particle trains, important for flagella maintenance and assembly, are observed to decrease in size as a function of cilia length.

Epidermal progenitors give rise to Merkel cells during embryonic development and adult homeostasis

Mon, 05 Oct 2009 12:07:45 PDT

Lineage-tracing experiments show that the origin of specialized mechanosensory Merkel cells in the skin is epidermal progenitors, not the neural crest.

Role of ERO1-{alpha}-mediated stimulation of inositol 1,4,5-triphosphate receptor activity in endoplasmic reticulum stress-induced apoptosis

Li, G., Mongillo, M., Chin, K.-T., Harding, H., Ron, D., Marks, A. R., Tabas, I. — Mon, 21 Sep 2009 08:52:26 PDT

CHOP turns on ERO1- to release calcium via IP3R and trigger cell death in response to ER stress.

Coassembly of Mgm1 isoforms requires cardiolipin and mediates mitochondrial inner membrane fusion

Mon, 21 Sep 2009 08:52:26 PDT

The soluble and membrane-anchored isoforms of Mgm1 are only active when they work together (in trans).

The dynamin-related GTPase Drp1 is required for embryonic and brain development in mice

Mon, 21 Sep 2009 08:52:26 PDT

Brain-specific Drp1 knockout mice demonstrate that Drp1-mediated organelle division is important for development, mitochondrial morphogenesis, and apoptosis.

Regulation of dynein-driven microtubule sliding by the axonemal protein kinase CK1 in Chlamydomonas flagella

Gokhale, A., Wirschell, M., Sale, W. S. — Mon, 21 Sep 2009 08:52:26 PDT

CK1 puts the brakes on dynein activity when added to purified axonemes in vitro, presumably to regulate how flagella bend.