The Journal of Cell Biology current issue

Niche recycling through division-independent egress of hematopoietic stem cells

Mon, 16 Nov 2009 10:11:50 PST

Transcellular migration of leukocytes is mediated by the endothelial lateral border recycling compartment

Mamdouh, Z., Mikhailov, A., Muller, W. A. — Mon, 16 Nov 2009 10:11:50 PST

Histone H2AX stabilizes broken DNA strands to suppress chromosome breaks and translocations during V(D)J recombination

Mon, 16 Nov 2009 10:11:50 PST

Cohesin pathway makes the necessary arrangements

Short, B. — Mon, 16 Nov 2009 10:11:49 PST

Germ cells decide for themselves

Short, B. — Mon, 16 Nov 2009 10:11:49 PST

Sizing up the ER stress response

Short, B. — Mon, 16 Nov 2009 10:11:49 PST

Adhering to the message

Short, B. — Mon, 16 Nov 2009 10:11:49 PST

How cell adhesion molecules transmit signals across the plasma membrane.

Michael Buszczak: Tracking the big game in stem cell identity

Sedwick, C. — Mon, 16 Nov 2009 10:11:49 PST

Buszczak is exploring the regulation of proteins that control stem cell identity in the fly.

Journeys through the Golgi--taking stock in a new era

Mon, 16 Nov 2009 10:11:49 PST

The Golgi apparatus is essential for protein sorting and transport. Many researchers have long been fascinated with the form and function of this organelle. Yet, despite decades of scrutiny, the mechanisms by which proteins are transported across the Golgi remain controversial. At a recent meeting, many prominent Golgi researchers assembled to critically evaluate the core issues in the field. This report presents the outcome of their discussions and highlights the key open questions that will help guide the field into a new era.

Cohesinopathy mutations disrupt the subnuclear organization of chromatin

Mon, 16 Nov 2009 10:11:50 PST

Nuclear morphology, chromosomal condensation, and transcriptional-mediated localization of genes to the nuclear periphery are disturbed by mutations in cohesin pathway genes.

A dual function for Pex3p in peroxisome formation and inheritance

Munck, J. M., Motley, A. M., Nuttall, J. M., Hettema, E. H. — Mon, 16 Nov 2009 10:11:50 PST

Pex3p interacts with peroxisome retention factor Inp1p at the peroxisomal membrane and functions in the organelle’s segregation in addition to its biogenesis.

Drosophila glypicans regulate the germline stem cell niche

Hayashi, Y., Kobayashi, S., Nakato, H. — Mon, 16 Nov 2009 10:11:50 PST

Heparan sulfate glycoproteins dally and dally-like define the germ cell niche in female and male Drosophila, respectively.

Quantitative analysis of chromatin compaction in living cells using FLIM-FRET

Lleres, D., James, J., Swift, S., Norman, D. G., Lamond, A. I. — Mon, 16 Nov 2009 10:11:50 PST

FRET analysis of cell lines expressing fluorescently tagged histones on separate nucleosomes demonstrates that variations in chromosome compaction occur during mitosis.

Lte1 contributes to Bfa1 localization rather than stimulating nucleotide exchange by Tem1

Geymonat, M., Spanos, A., de Bettignies, G., Sedgwick, S. G. — Mon, 16 Nov 2009 10:11:50 PST

Reevaluation of Lte1’s involvement in the mitotic exit network reveals that it is involved in localizing Bfa1 to the spindle pole body but does not function as a GEF for Tem1.

Capacity for stochastic self-renewal and differentiation in mammalian spermatogonial stem cells

Mon, 16 Nov 2009 10:11:50 PST

Spermatogonial stem cells have an innate ability to choose, with constant probability, between different fates independently of cues from the microenvironment.

Membrane expansion alleviates endoplasmic reticulum stress independently of the unfolded protein response

Schuck, S., Prinz, W. A., Thorn, K. S., Voss, C., Walter, P. — Mon, 16 Nov 2009 10:11:50 PST

Increasing the size of the ER by lipid synthesis helps the cell deal with ER stress.

Adenovirus RID-{alpha} activates an autonomous cholesterol regulatory mechanism that rescues defects linked to Niemann-Pick disease type C

Cianciola, N. L., Carlin, C. R. — Mon, 16 Nov 2009 10:11:50 PST

Viral subversion of cholesterol homeostasis provides insights into sterol trafficking, autophagy, and lysosomal storage diseases.

The CEACAM1 N-terminal Ig domain mediates cis- and trans-binding and is essential for allosteric rearrangements of CEACAM1 microclusters

Mon, 16 Nov 2009 10:11:50 PST

Structural analyses reveal that oligomerization between cell adhesion molecules in the same membrane is influenced by their interactions across opposing membranes (see also in this issue the accompanying paper by Müller et al.).

Homophilic adhesion and CEACAM1-S regulate dimerization of CEACAM1-L and recruitment of SHP-2 and c-Src

Muller, M. M., Klaile, E., Vorontsova, O., Singer, B. B., Obrink, B. — Mon, 16 Nov 2009 10:11:50 PST

The monomer/dimer equilibrium of adhesion molecule CEACAM1-L is regulated by binding between opposing membranes, which in turn controls cytoplasmic enzyme binding and signaling (see also in this issue the accompanying paper by Klaile et al.).