The Journal of Cell Biology current issue

Runx proteins regulate Foxp3 expression

Mon, 02 Nov 2009 10:42:11 PST

Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow

Mon, 02 Nov 2009 10:42:11 PST

Spermatozoa capture HIV-1 through heparan sulfate and efficiently transmit the virus to dendritic cells

Mon, 02 Nov 2009 10:42:11 PST

{gamma}-TuRC gives microtubules pause for thought

Short, B. — Mon, 02 Nov 2009 10:42:10 PST

Raf-1 sheds then spreads its inhibitions

Short, B. — Mon, 02 Nov 2009 10:42:10 PST

Chemosensitivity depends on the dependency

Short, B. — Mon, 02 Nov 2009 10:42:10 PST

Not the average view of adipogenesis

Short, B. — Mon, 02 Nov 2009 10:42:10 PST

Study on cell heterogeneity offers a surprising twist to adipocyte differentiation.

Irving Weissman: Working for regenerative medicine

LeBrasseur, N. — Mon, 02 Nov 2009 10:42:10 PST

A force behind both the science and politics of stem cell biology, Weissman now strives to translate his successes to the clinic.

The ubiquitin landscape at DNA double-strand breaks

Messick, T. E., Greenberg, R. A. — Mon, 02 Nov 2009 10:42:10 PST

The intimate relationship between DNA double-strand break (DSB) repair and cancer susceptibility has sparked profound interest in how transactions on DNA and chromatin surrounding DNA damage influence genome integrity. Recent evidence implicates a substantial commitment of the cellular DNA damage response machinery to the synthesis, recognition, and hydrolysis of ubiquitin chains at DNA damage sites. In this review, we propose that, in order to accommodate parallel processes involved in DSB repair and checkpoint signaling, DSB-associated ubiquitin structures must be nonuniform, using different linkages for distinct functional outputs. We highlight recent advances in the study of nondegradative ubiquitin signaling at DSBs, and discuss how recognition of different ubiquitin structures may influence DNA damage responses.

{gamma}-Tubulin ring complexes regulate microtubule plus end dynamics

Mon, 02 Nov 2009 10:42:10 PST

Independently of their nucleation activity, -tubulin ring complex proteins localize along microtubules, limiting catastrophe events during interphase.

From autoinhibition to inhibition in trans: the Raf-1 regulatory domain inhibits Rok-{alpha} kinase activity

Mon, 02 Nov 2009 10:42:10 PST

The mechanism by which Raf-1 antagonizes Rok- to promote migration and tumorigenesis is revealed.

Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1

Mon, 02 Nov 2009 10:42:10 PST

Cripto-1 associates with Notch1 in the endoplasmic reticulum and Golgi to enhance Notch1 localization to lipid rafts and its maturation.

Ana3 is a conserved protein required for the structural integrity of centrioles and basal bodies

Stevens, N. R., Dobbelaere, J., Wainman, A., Gergely, F., Raff, J. W. — Mon, 02 Nov 2009 10:42:10 PST

Centriolar protein Ana3 is essential for basal body formation, not centriole duplication, as previously thought.

Lateral transport of Smoothened from the plasma membrane to the membrane of the cilium

Milenkovic, L., Scott, M. P., Rohatgi, R. — Mon, 02 Nov 2009 10:42:10 PST

A distinct pathway for ligand-stimulated lateral trafficking of the hedgehog protein Smoothened into cilia is described.

Heterogeneity in the physiological states and pharmacological responses of differentiating 3T3-L1 preadipocytes

Loo, L.-H., Lin, H.-J., Singh, D. K., Lyons, K. M., Altschuler, S. J., Wu, L. F. — Mon, 02 Nov 2009 10:42:11 PST

A systems biology–based analysis shows that differentiating adipocytes look very different at the single-cell level and form distinct cellular subpopulations.

Mammalian Rif1 contributes to replication stress survival and homology-directed repair

Buonomo, S. B.C., Wu, Y., Ferguson, D., de Lange, T. — Mon, 02 Nov 2009 10:42:11 PST

Multifunctional protein Rif1 accumulates at stalled replication forks to facilitate DNA repair during S phase.

Ase1/Prc1-dependent spindle elongation corrects merotely during anaphase in fission yeast

Courtheoux, T., Gay, G., Gachet, Y., Tournier, S. — Mon, 02 Nov 2009 10:42:11 PST

The tug of war that ensues when a kinetochore binds microtubules from both spindle poles is resolved by Ase1/Prc1.

Membrane proteins Bqt3 and -4 anchor telomeres to the nuclear envelope to ensure chromosomal bouquet formation

Mon, 02 Nov 2009 10:42:11 PST

A screen identifies two more bouquet proteins required for meiotic telomere clustering: Bqt4 anchors the telomeres, whereas Bqt3 protects Bqt4 from degradation.

MCL-1-dependent leukemia cells are more sensitive to chemotherapy than BCL-2-dependent counterparts

Brunelle, J. K., Ryan, J., Yecies, D., Opferman, J. T., Letai, A. — Mon, 02 Nov 2009 10:42:11 PST

Cancer-expressed anti-apoptotic protein BCL-2 is stable, whereas MCL-1 undergoes proteasome-dependent degradation in response to chemotherapeutic agents.